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    Detection of Proteins Directions for Use: Additionally, it rencojtre recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. Wrap in plastic wrap and expose to x-ray film. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.

    Bring to ml with deionized H Primary Antibody Dilution Buffer: